Protease and Cellulase enzyme from Bacillus sp. (Liquid)
A cell-free microbial biostimulant in liquid form, notified under FCO Schedule VI. Key notified measures: Total organic carbon ≥ 1.22 % w/w; Solubility ≥ 95.07 % w/w. Notified for groundnut.
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Composition
As notified in the Gazette, ingredient by ingredient.
| Ingredient | Content | Limit | |
|---|---|---|---|
| i | Protease enzyme (source: microbial fermentation of Bacillus species) 25 HUT/ml per cent. by volume, minimum | 0.5% v/v | Minimum |
| ii | Cellulase enzyme (source: microbial fermentation of Bacillus species) (500 EU/ml) per cent. by volume, minimum | 1% v/v | Minimum |
| iii | IAA ppm (From microbial source) per cent. by volume, minimum | 0.0008ppm | Minimum |
| iv | Methylisothiazolinone as preservative per cent. by weight, minimum | 0.1% w/w | Minimum |
| v | Water per cent. by volume | QS% v/v | Exact / specified |
| vi | Total (per cent.) | 100 | Exact / specified |
Specifications (label claims)
The parameters a sample must meet, with the Part D method used to test each one.
| Parameter | Notified value | Test method | |
|---|---|---|---|
| i | Protease enzyme (HUT/ml) | 25 | Method 22: Estimation of protease activity |
| ii | Cellulase enzyme (EU/ml) | 500 | Method 37: Estimation of cellulase activity |
| iii | pH (10% aqueous solution) | 4.5- 6.0pH | Method 1: Estimation of pH |
| iv | Specific gravity | 1.010 ± 0.02Specific gravity | Method 2: Estimation of specific gravity |
| v | Total organic carbon per cent. by weight, minimum | 1.22% w/w | Method 4: Estimation of organic matter and organic carbon |
| vi | Solubility per cent. by weight, minimum | 95.07% w/w | Method 5: Estimation of water solubility |
Crops and doses
The Gazette wording is what counts; per-acre figures use 1 hectare = 2.47105 acres.
| Crop | Gazette dose | Per acre, each application | Per acre, season |
|---|---|---|---|
| Groundnut | Two soil applications at 5 litre/ha | 2.02 litre | 4.05 litre |
Notification history
- 13 Aug 2025S.O. 3741(E): entry inserted
Official notifications: Ministry of Agriculture & Farmers Welfare, biostimulant circulars.
Before you buy or stock it
- Check that the product name, composition and crop on the pack match this page.
- Use only the notified dose for your crop; the calculator converts it to your area.
- Ask the seller for the batch number and expiry date, and keep the bill.
Questions about your crop? Ask our agri expert on WhatsApp.
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Consolidated by NanoKheti from the Gazette of India notifications under Schedule VI of the Fertiliser (Inorganic, Organic or Mixed) (Control) Order, 1985, for reference. The Gazette text is authoritative; this page is not legal advice. Check the notification before labelling, licensing or buying. Covers notifications up to S.O. 1574(E) (25 Mar 2026); checked for later Schedule VI changes on 19 October 2026. Questions? Ask our agri expert on WhatsApp.