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NanoKhetiArticle 05 · Biostimulants
Innovations & Patents
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  1. Key Points
  2. Biostimulant or Fertilizer
  3. Findings
  4. Limits
  5. How To Use
  6. Bollworm
  7. FAQ
  8. Sources
Field Note 05 · Biostimulants

Chitosan for crops: what a natural molecule from prawn shells can do

Chitosan is sold to farmers as a biostimulant. Here is what it is, what research shows it can and cannot do, what a cotton trial reported, and how to use it wisely.

Key points

  • Chitosan is a natural molecule made from crab and prawn shells. In plants it works mainly as a "wake-up signal" that switches on the crop's own defences, and it can support growth.
  • It is a biostimulant, not a fertilizer and not a registered insecticide. Use it alongside good nutrition and pest management, not instead of them.
  • In a cotton trial co-authored by Dr. Mathur, sprayed plots were reported taller, with more leaves and about a third fewer bollworms. These figures come from the five best plots, and yield was not measured.
  • Buy a registered product, follow its label dose, and compare it against an unsprayed strip in your own field.

What is chitosan?

Crabs, prawns and shrimps carry their skeleton on the outside, as a hard shell. That shell is made largely of chitin, one of the most common natural materials on earth. Insects and many fungi are built from it too.

When chitin from seafood waste is cleaned and treated with a strong alkali, it becomes chitosan. Break chitosan into short pieces and you get chito-oligosaccharides (COS), which dissolve more easily in water.

So chitosan turns a waste product of the fishing industry into a farm input. It is biodegradable and breaks down in soil.

Biostimulant or fertilizer: what's the difference?

Chitosan is a biostimulant. That word is now common on product labels, and it is often confused with fertilizer.

Fertilizer vs biostimulant
FeatureFertilizerBiostimulant
What it doesSupplies the nutrients a crop needs (nitrogen, phosphorus, potassium, micronutrients)Helps the crop take up and use nutrients, and cope with stress such as heat, drought or disease pressure
How much is usedKilograms per acreSmall doses, usually a few grams or millilitres per litre of spray
ExamplesUrea, DAP, potash, farmyard manureSeaweed extracts, humic acids, amino acids, chitosan
Can one replace the other?NoNo

A biostimulant cannot feed a hungry crop. A well-fed crop, though, can get more out of each bag of fertilizer when a good biostimulant helps it. They work best together.

Since 2021, biostimulants sold in India have been brought under the Fertiliser Control Order (FCO), and products must be approved before they can be sold. Before you buy, check the pack for registration details.

How chitosan helps plants: what research shows

Scientists have studied chitosan in crops for decades. Reviews of this research describe a few main effects:

  • It switches on the plant's defences. The plant senses chitosan much as it would sense a fungus attacking it. It responds by strengthening its cell walls and making its own protective compounds. This "priming" helps it resist disease that arrives later.
  • It acts directly against some fungi and bacteria, slowing their growth.
  • It can support growth and stress tolerance. Studies report effects on leaf pores (stomata), which can reduce water loss. It has also been tested as a seed coating and as a coating that keeps harvested fruit fresh for longer.

Results vary with the crop, the dose, the timing and the type of chitosan used. Chitosan is a helper, not a cure-all.

What the cotton study tested

The study was published in 2021 in the Journal of Advanced Scientific Research. Its authors are Manjit Kaur and Sanjeev Kalia of RIMT University, Punjab, and Dr. Abhishek Mathur, then at the R&D department of Prathista Industries, Telangana. Dr. Mathur was the third author.

The team did three things:

  1. Made chitosan and COS from crab and prawn shell waste in the laboratory.
  2. Tested COS against three crop fungi on lab plates: the fungi behind wilt (Fusarium), collar rot (Sclerotium) and anthracnose (Colletotrichum) in many crops.
  3. Sprayed cotton in field plots with a chitosan solution at 5 ml per litre and compared them with unsprayed plots. Each plot had 25 plants, both sets got cow-dung manure, and the crop was measured after two months.

What they found

Plant height · cm
Unsprayed15 cm
Chitosan spray56 cm
Leaves per plant
Unsprayed22
Chitosan spray33
Fall in bollworm numbers · vs unsprayed
Chitosan spray33%
Fig. 1Cotton height, leaves and bollworm reduction with and without chitosan spray, after two months. Averages of the five best plots, as reported. Data: Kaur et al., 2021. Chart: NanoKheti.
Cotton after two months
MeasureUnsprayedSprayed with chitosan
Plant height15 cm56 cm
Leaves per plant2233
Fall in bollworm numbers—33%

Averages of the five best plots, as reported in the paper.

  • In the field, sprayed cotton was reported much taller, with half as many leaves again, and with about a third fewer bollworms. The paper does not say which bollworm (pink, American or spotted).
  • In the lab, COS slowed all three fungi. It was less strong than the comparison, an anti-fungal medicine used for people.

In simple words: the signs are promising, and they match what other research says chitosan can do. But this one trial cannot tell you how much chitosan will do in your field.

What this study does not tell us

  • The field figures are the averages of the five best plots, not of all plots. They show chitosan at its best, not on an average field.
  • Yield was not measured. We don't know whether the taller plants gave more cotton.
  • No statistics were reported for the field results.
  • Key details are missing: where and when the trial was run, the cotton variety, how many times it was sprayed, the strength of the chitosan solution, and which bollworm was counted.
  • The fungus test was on lab plates only, not on diseased plants in a field.

The study is a useful early signal. It is not proof that chitosan will control bollworm or raise yield.

Using chitosan on your farm sensibly

  1. Buy a registered product and follow its label dose. You cannot copy this study's "5 ml per litre", because it does not say how strong its chitosan solution was.
  2. Spray early. Chitosan primes the plant's defences, so it helps most before disease or pests build up. Follow the crop stages given on the label.
  3. Check the label before mixing chitosan with other sprays.
  4. Keep the crop well fed. Chitosan adds almost no nutrients. Base your fertilizer on a soil test.
  5. Test it yourself. Leave one strip of the field unsprayed and compare growth, pest damage and yield at the end of the season.

Bollworm in cotton: what ICAR-CICR advises

Products sold in India for pest control must be registered with the Central Insecticides Board. Chitosan is not a replacement for proper bollworm management. ICAR's Central Institute for Cotton Research (ICAR-CICR), Nagpur, advises cotton farmers to:

  • Monitor with pheromone traps, about 5 per hectare, from around 45 days after sowing.
  • Remove and destroy rosette flowers (twisted, half-open flowers that hide pink bollworm larvae).
  • Check about 20 green bolls per acre for larvae and damage.
  • Sow on time, using short- to medium-duration Bt hybrids recommended for your region; rotate crops and don't stretch the cotton season.
  • Spray a registered insecticide only when monitoring shows it is needed, as your KVK or agriculture officer advises.

What comes next

Dr. Mathur has worked on chitin and chitosan for more than a decade, as part of his wider work on natural molecules for farming. As he puts it: "Natural molecules are the future for tomorrow."

NanoKheti does not sell a chitosan product. This article is here to help you judge the chitosan products you see in the market.

Questions · Chitosan

Frequently Asked Questions

Is chitosan a fertilizer?

No. Chitosan is a biostimulant: it helps the plant defend itself and use nutrients better, but it supplies almost no nutrients. Your crop still needs balanced fertilizer.

How do I use chitosan on crops?

Use a registered product and follow the dose and timing on its label. It is usually sprayed on leaves or used to treat seed. Spray early, before pest or disease pressure builds up.

Can chitosan control bollworm?

One small trial reported about a third fewer bollworms on sprayed cotton, in its best plots. That is not enough to rely on. Follow ICAR-CICR's bollworm advice, and use registered insecticides when monitoring shows they are needed.

Is chitosan natural and safe to use?

Chitosan is made from natural shell material, breaks down in soil and is generally regarded as low in toxicity. Commercial products contain other ingredients too, so always follow the safety advice on the label.

Does NanoKheti sell chitosan?

No. For advice on your crop, message our agri experts on WhatsApp.

Sources · About the researcher

Sources

  1. Kaur, M., Kalia, S., & Mathur, A. (2021). Chitosan and chito-oligosaccharides extracted and purified from exoskeletons of crustaceans: potent growth promotional and pesticidal activities on cotton crop. Journal of Advanced Scientific Research, 12(2) Suppl 1, 220–224.sciensage.info/index.php/JASR/article/view/1155
  2. Stasińska-Jakubas, M., & Hawrylak-Nowak, B. (2022). Protective, biostimulating, and eliciting effects of chitosan and its derivatives on crop plants. Molecules, 27(9), 2801.https://doi.org/10.3390/molecules27092801
  3. ICAR-Central Institute for Cotton Research, Nagpur. Pest and disease management advisory (pink bollworm).cicr.org.in
  4. Government of India. Biostimulants under the Fertiliser Control Order, 1985 (notification S.O. 882(E), 23 February 2021).pib.gov.in

This is an original plain-language summary written by NanoKheti. The paper's text, tables and figures are not reproduced here.

About the researcher

Dr. Abhishek Mathur

Board Advisor · Innovation & Patents

NanoKheti's Board Advisor for Innovation & Patents. An agricultural biotechnologist with more than 20 years in research and industry, he works on bio-fertilizers, bio-stimulants and natural molecules for crop protection.

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